Virender Sehwag replaced Yousuf Pathan in the Indian side for the one-off Twenty20 international, while Murali Kartik came in for all-rounder Joginder Sharma.
The world champions, having already sealed the series 4-1, fielded an unchanged side after opener Matthew Hayden failed to recover fully from his hip injury.
India made one change, bringing in leg-spinner Piyush Chawla in place of Munaf Patel for the first of the best of three finals.
After Hayden, Gambhir is perched at the second spot by accumulating 227 runs at 37.83 in the batsmen's list.
Brett Lee claimed the first hat-trick as Australia thrashed Bangladesh by nine wickets in a Super Eight game of the Twenty20 World Cup.
India recalled Virender Sehwag for Yuvraj Singh and dropped spinner Harbhajan Singh for paceman Irfan Pathan.
The second Test against India starts in Adelaide on Friday.
The India captain said his five-wicket haul on the opening day of the first Test against Australia is the best of his career.
News of all that's transpired on and off the football field
Australia's Phil Jaques will replace the retired Justin Langer in the first Test against Sri Lanka.
Brighton and Hove Albion's Tomer Hemed grabbed the only goal as they ended Newcastle United's three-match winning streak with a 1-0 victory on Sunday in a battle between two of this season's promoted sides.
The Aussies won the Group B match at the Twenty20 World Cup by 8 wickets and entered the Super Eight stage.
Australia beat New Zealand by four wickets in the warm-up match ahead of the Twenty20 World Cup.
In the ICC ODI team rankings, India remains in fifth place.
The Kiwis scored a thrilling one-wicket win in the third and final one-day match of the Chappell-Hadlee series.
The International Cricket Council announced on Monday that Jamtha Stadium, Nagpur, has been given an official warning under the ICC Pitch Monitoring Process following the third Test between India and South Africa.
Finding massive irregularity during "standard fraud checks", the White House has removed at least 85,000 signatures from an on-line petition which asked US President Barack Obama to cancel his planned meeting with Prime Minister Narendra Modi next month.
Veteran Jorge Lombi scored a hat-trick as Argentina beat New Zealand 3-2 to win the Champions Challenge men's hockey tournament.
Former world champion Valentino Rossi won the MotoGP at the Dutch Grand Prix after a 15 lap battle with overall leader Casey Stoner on Saturday.
New Zealand beat India 2-0 in the opening match of the Champions Challenge.
UEFA said it would replace the pitch at the Stade Pierre Mauroy in Lille after Wednesday's final group E match between Italy and Ireland.
In major embarrassment for the Board of Control for Cricket in India, the International Cricket Council rated the track at the Vidarbha Cricket Association ground in Nagpur, on which the third Test between India and South Africa was played, as "poor", triggering a review of the performance of the pitch under the ICC Pitch Monitoring Process.
Matthew Hoggard took four wickets to reduce Australia to 312 for five on Day 3 of the Ashes Test.
A detailed statistical analysis of the four-match series.
South African cricket captain AB de Villiers continued to script statistical history at the ongoing World Cup as he entered the top-10 of all-time scorers' list.
For those who have accepted without question the propaganda pedalled about Sourav Ganguly in recent years this will come as quite a surprise.
West Indies brought back fast bowlers Jerome Taylor and Fidel Edwards in place of Marlon Samuels and Corey Collymore.
Australia's senior cricketers are playing down suggestions they might retire en masse if they win back the Ashes.
Tendulkar's ranking may have slipped but he will show why he is recognised as the world's best batsman in the remaining three Tests, Indian teammate VVS Laxman said on Tuesday.
England ended a run of nine straight losses to Australia with a stunning 92-run win.
Italian Valentino Rossi extended his contract with Yamaha to the end of 2008.
Italian Loris Capirossi won the MotoGP Czech Grand Prix after leading from start to finish.
Australia beat New Zealand by eight runs a high-scoring tri-series ODI.
Set a target of 243, Australia chased down the total with 4.4 overs to spare in the tri-series opener that also involves New Zealand.
"I would like to emulate kaka and become a master-blaster like him," said Rohan Tendulkar, nephew of Sachin Tendulkar.
Australia beat England by 10 wickets to win the fifth Test and complete their first Ashes series whitewash in 86 years.
The Italian kept alive the defence of his MotoGP title with a battling victory in the German Grand Prix.
The Italian Yamaha rider fought from 11th position at the start for his second straight win at the twisting Sachsenring circuit.
Sehwag, however, could not improve on his seventh position.
